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Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis
DOI:

09:26 min

March 23, 2021

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Chapters

  • 00:04Introduction
  • 00:26Tissue Crosslinking
  • 02:24Chromatin Isolation
  • 03:26DNA Purification
  • 06:50Results: ChIP‐qPCR and ChIP‐Seq Analysis
  • 08:52Conclusion

Summary

Automatic Translation

This protocol focuses on chromatin preparation from snap frozen tissues and it is suitable for Crosslinking Chromatin Immunoprecipitation (X-ChIP) followed by either quantitative PCR analysis (X-ChIP-qPCR) or next generation sequencing approaches (X-ChIP-seq).

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