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Biology

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Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
 

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Article DOI: 10.3791/50064 14:29 min
May 1st, 2013

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Summary May 1st, 2013

We describe a robust method for chromatin immunoprecipitation using primary T cells. The method is founded on standard approaches, but uses a specific set of conditions and reagents that improve efficiency for limited a quantities of cells. Importantly, a detailed description of the data analysis phase is presented.

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