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Journal / Immunology and Infection / Monitoreo De La ElastasA NeutrófiloS Y La…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Monitoreo De La ElastasA NeutrófiloS Y La Actividad De La Catepsina G En Muestras De Esputo Humano
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Monitoreo De La ElastasA NeutrófiloS Y La Actividad De La Catepsina G En Muestras De Esputo Humano

Article DOI: 10.3791/62193-v • 09:23 min • May 21st, 2021
May 21st, 2021 •
*1,2, *1,2,3,4, 1,2,5,6,7, 1,3,8
1Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), 2Dept. of Translational Pulmonology, University of Heidelberg, 3Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL), University of Heidelberg, 4Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University, University of Heidelberg, 5Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine, Charité – Universitätsmedizin Berlin, 6Berlin Institute of Health (BIH), 7German Center for Lung Research (DZL), Associated Partner Site, Berlin, 8Dept. of Chemical Physiology and Biochemistry, Oregon Health and Science University
* These authors contributed equally

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Los protocolos aquí descritos proporcionan una guía para visualizar y cuantificar la actividad de las proteasas de neutrófilos en el esputo humano. Las aplicaciones de este tipo de análisis abarcan desde la evaluación de tratamientos antiinflamatorios hasta la validación de biomarcadores, el cribado de fármacos y los estudios clínicos de cohortes grandes.

Tags

Inmunología e Infección Número 171 Fibrosis quística enfermedad pulmonar inflamación proteasas reporteros fret elastasa neutrófila catepsina G diagnóstico
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