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March 23, 2021
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This protocol describes a method of generating large volumes of lipid encapsulated decafluorobutane microbubbles using probe-tip sonication and subsequently condensing them into phase-shift nanodroplets using high-pressure extrusion and mechanical filtration.
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Cite this Article
Merillat, D. A., Honari, A., Sirsi, S. R. Production of Membrane-Filtered Phase-Shift Decafluorobutane Nanodroplets from Preformed Microbubbles. J. Vis. Exp. (169), e62203, doi:10.3791/62203 (2021).
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