Journal
/
/
Önceden Biçimlendirilmiş Mikrobubbles'tan Membran Filtreli Faz KaydırmaLı Decafluorobutane Nanodroplets Üretimi
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Production of Membrane-Filtered Phase-Shift Decafluorobutane Nanodroplets from Preformed Microbubbles
DOI:

07:10 min

March 23, 2021

, ,

Chapters

  • 00:04Introduction
  • 00:46Generating Microbubbles from Lipid Films
  • 01:48Preparing and Priming the Extruder for Microbubble Condensation
  • 03:13Pre-Cooling Microbubbles for Extrusion and Separating Droplets from Liposomes by Centrifugation
  • 05:04Results: Phase-Shift Droplet Analysis of DFB and OFP Nanodroplets Using DLS, TRPS and Microscopy
  • 06:19Conclusion

Summary

Automatic Translation

Bu protokol, prob ucu sonikasyonu kullanarak büyük hacimlerde lipid kapsüllenmiş decafluorobutane mikrobubbles üretme ve daha sonra yüksek basınçlı ekstrüzyon ve mekanik filtrasyon kullanarak faz kaydırma nanodropletlerine yoğuşma yöntemini açıklar.

Related Videos

Read Article