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Production and Targeting of Monovalent Quantum Dots

Production and Targeting of Monovalent Quantum Dots

Article DOI: 10.3791/52198-v 10:16 min October 23rd, 2014
October 23rd, 2014



We provide detailed instructions for the preparation of monovalent targeted quantum dots (mQDs) from phosphorothioate DNA of defined length. DNA wrapping occurs in high yield, and therefore, products do not require purification. We demonstrate the use of the SNAP tag to target mQDs to cell-surface receptors for live-cell imaging applications.


Production Targeting Monovalent Quantum Dots Multivalent Quantum Dots CdSe/ZnS Core/shell QDs Phosphorothioate DNA PtDNA Imaging Probes SNAP-tagged Notch Receptors Live Mammalian Cells Benzylguanine Moiety
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