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1Department of Otolaryngology,University of California, San Francisco, 2Department of Chemistry,University of California, Berkeley, 3Materials Science Division,Lawrence Berkeley National Laboratory, 4Department of Pharmaceutical Chemistry,University of California, San Francisco, 5Tetrad Graduate Program,University of California, San Francisco, 6Center for Systems and Synthetic Biology,University of California, San Francisco, 7Chemistry and Chemical Biology Graduate Program,University of California, San Francisco
Chapters
- 00:05Title
- 02:38Phase Transfer of Quantum Dots from Organic to Aqueous Phase
- 04:21Preparation of Monovalent Targeted Quantum Dots
- 06:43Labeling Live Cells with Monovalent Quantum Dots
- 08:06Results: Production of Monovalent Quantum Dots and Labeling of SNAP-tagged Proteins on Live Cells
- 09:15Conclusion
We provide detailed instructions for the preparation of monovalent targeted quantum dots (mQDs) from phosphorothioate DNA of defined length. DNA wrapping occurs in high yield, and therefore, products do not require purification. We demonstrate the use of the SNAP tag to target mQDs to cell-surface receptors for live-cell imaging applications.
