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JoVE Journal Developmental Biology
Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome
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Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome

Article DOI: 10.3791/62240-v • 09:39 min • December 4th, 2021
December 4th, 2021 •
*1, *1, 2, 1, 1
1Manipal Institute of Regenerative Medicine, Manipal Academy of Higher Education, Manipal, Karnataka, India-576104, 2Department of Medical Genetics, Manipal Hospitals
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes the generation of integration free iPSCs from fetal tissue fibroblasts through delivery of episomal plasmids by nucleofection followed by description of methods used for iPSC characterization and neuronal differentiation.

Tags

Turner Syndrome 45XO Induced Pluripotent Stem Cells Fetal Cells Neurological Deficits Cell Modelling Aneuploid Fetuses IPSC Generation Perpetual Conservation Embryonic Development Episomal Reprogramming Plasmids Nuclear Fiction Reprogrammed IPSCs Fetal Chorionic Villi Collagenase Blend
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