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成人筋幹細胞機能評価のための単一筋繊維培養アッセイ Ex Vivo
JoVE Journal
Biology
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JoVE Journal
Biology
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
Please note that all translations are automatically generated.
Click here for the English version.
成人筋幹細胞機能評価のための単一筋繊維培養アッセイ Ex Vivo
DOI:
10.3791/62257-v
•
09:19 min
•
February 15, 2021
•
Sören S. Hüttner
,
Christian Hayn
,
Hellen E. Ahrens
,
Manuel Schmidt
,
Henriette Henze
,
Julia von Maltzahn
1
Leibniz Institute on Aging, Fritz-Lipmann-Institute
Chapters
00:04
Introduction
00:50
Preparing Pipettes for Trituration of the Collagenase-Digested Muscles
01:54
EDL Muscle Isolation and Collagenase Digestion
03:19
Myofiber Dissociation and Culture
04:20
siRNA Transfection, Fixation, and IF Staining
06:31
Results: Culture and Transfection of Murine EDL Muscle-Derived Single Myofibres
08:34
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルでは、筋肉幹細胞のインビトロ培養および機能解析方法が記載されており、内因性ニッチとの相互作用のほとんどを維持する。
Tags
Muscle Stem Cells
Single Myofiber Culture
Ex Vivo
Niche
SiRNA Transfection
Pasteur Pipettes
Horse Serum
Ethanol
Tibialis Anterior
EDL Muscle
Digestion
Isolation Medium
Myofibers
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