Isolation, Culture, and Transplantation of Muscle Satellite Cells

The overall goal of this procedure is to transplant purified, quiescent adult neuron skeletal muscle satellite cells for the treatment of skeletal muscle injury. This is accomplished by first dissecting skeletal muscle from adult mice and enzymatically digesting skeletal muscle, and then sorting the quiescent satellite cells by magnetic bead separation. Next, the isolated cells are expanded on collagen coated culture dishes, and in the final step, the in vitro expanded cells are injected into the cardio toin treated skeletal muscles of adult mice.

Ultimately, the engraftment and contribution of in vitro expanded satellite cells to the regeneration of muscle fibers can be assessed by xal staining. Hi, my name is a sushi ura. I'm associate professor in the Stem Cell Institute, university of Minnesota.