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JoVE Journal
Biochemistry

This content is Open Access.

基于核磁共振的片段筛选,样品最少,自动化程度最高
 
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基于核磁共振的片段筛选,样品最少,自动化程度最高

Article DOI: 10.3791/62262-v 09:19 min June 4th, 2021
June 4th, 2021

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

核磁共振基于片段的筛选是快速鉴定生物大分子(DNA、RNA或蛋白质)的小分子结合剂的可靠方法。介绍了描述基于自动化的样品制备、NMR 实验和采集条件以及分析工作流程的协议。该技术允许对 1H和 19F NMR活性细胞核进行最佳利用以进行检测。

Tags

本月在JoVE上,第172期,基于片段的筛选,自动化,溶液核磁共振波谱,数据收集和分析,筛选平台,片段库
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