Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

多参数灌注对照分离原代大鼠肝细胞
 
Click here for the English version

多参数灌注对照分离原代大鼠肝细胞

Article DOI: 10.3791/62289-v 07:35 min April 5th, 2021
April 5th, 2021

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

该协议详细说明了使用特殊的静脉内导管,标准化的无菌一次性导管,通过实时监测补充的温度控制,以及用于两步胶原酶灌注手术的报警系统,以提高分离的原代大鼠肝细胞的活力,产量和功能的一致性。

Tags

生物学,第170期,细胞分离,肝细胞,两步,灌注,插管,集成装置
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter