Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolering av primära råtthepatocyter med…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Isolering av primära råtthepatocyter med multiparameterperfusionskontroll
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
Journal (Biology)
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
Knowing What Counts: Unbiased Stereology in the Non-human Primate Brain
Journal (Biology)
Knowing What Counts: Unbiased Stereology in the Non-human Primate Brain
 
Click here for the English version

Isolering av primära råtthepatocyter med multiparameterperfusionskontroll

Article DOI: 10.3791/62289-v • 07:35 min • April 5th, 2021
April 5th, 2021 •
*1, *2, 3, 4, 5, 1, 3, 1,3,4,5,6
1Department of Physiology & The Institute for Digital Medicine (WisDM), National University of Singapore, 2College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, 3Mechanobiology Institute, National University of Singapore, 4Institute of Bioengineering and Nanotechnology, A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering, 6CAMP, Singapore-MIT Alliance for Research and Technology
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Detta protokoll beskriver användningen av en speciell intravenös kateter, standardiserade sterila engångsrör, temperaturkontroll kompletterad med realtidsövervakning och ett larmsystem för tvåstegs kollagenasperfusionsförfarande för att förbättra konsistensen i livskraften, utbytet och funktionaliteten hos isolerade primära råtthepatocyter.

Tags

Biologi utgåva 170 cellisolering hepatocyt tvåstegs perfusion kannulering integrerad enhet
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter