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Immunology and Infection

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JoVE Journal Immunology and Infection
Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
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Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Article DOI: 10.3791/62387-v • 10:37 min • October 20th, 2021
October 20th, 2021 •
1,2, 1,2, 1,2, 1,2
1Department of Infectious Diseases, Southwest Hospital, Third Military Medical University (Army Medial University), 2Chongqing Key Laboratory for Research of Infectious Diseases

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Summary
Automatic Translation

Based on a hepatitis B virus (HBV)-derived peptide matrix, HBV-specific CD4 T-cell responses could be evaluated in parallel with identification of HBV-specific CD4 T-cell epitopes.

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HBV-specific CD4 T-cell Responses HLA-DR-restricted CD4 T-cell Epitopes Peptide Matrix Chronic HBV Infection Viral Clearance Peripheral Blood Mononuclear Cells (PBMCs) Ficoll Density Gradient Centrifugation Benzonase RPMI 1640 Cryo Vial Centrifuge Tube Cell Suspension Viable Cells Trypan Blue Hemocytometer Complete Culture Medium IL2 IL7
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