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Analyse av HBV-spesifikke CD4 T-celleresponser og identifisering av HLA-DR-begrensede CD4 T-celle epitoper basert på en peptidmatrise
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
DOI:

10:37 min

October 20, 2021

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Chapters

  • 00:04Introduction
  • 00:51Isolation of PBMCs and In Vitro Expansion Using an HBV Peptide Matrix
  • 03:55Analysis of HBV-specific CD4 T-Cell Responses by Intracellular Flow Cytometry
  • 05:54Identification of HBV-specific HLA-DR Restricted CD4 T-Cell Epitopes
  • 08:07Results: Identification of HBV-specific HLA-DR Restricted CD4 T-Cell Epitopes
  • 10:06Conclusion

Summary

Automatic Translation

Basert på en hepatitt B-virus (HBV)-avledet peptidmatrise, kan HBV-spesifikke CD4 T-celleresponser evalueres parallelt med identifisering av HBV-spesifikke CD4 T-celle epitoper.

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