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Isolering af primære ventilceller til in vitro-sygdomsmodellering
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Isolering af primære ventilceller til in vitro-sygdomsmodellering

Article DOI: 10.3791/62439-v • 07:31 min • April 16th, 2021
April 16th, 2021 •
1, 1, 1, 1, 2, 1,3
1Division of Cardiology, Department of Medicine, and the Pittsburgh Heart, Lung, and Blood Vascular Medicine Institute, University of Pittsburgh, 2Division of Cardiac Surgery, Department of Cardiothoracic Surgery, University of Pittsburgh and Heart and Vascular Institute, University of Pittsburgh Medical Center, 3Department of Bioengineering, University of Pittsburgh

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Denne protokol beskriver samlingen af humane aortaklapper ekstraheret under kirurgiske aortaklapudskiftningsprocedurer eller fra kadavervæv og den efterfølgende isolering, ekspansion og karakterisering af patientspecifikke primære ventilendotel- og interstitielle celler. Inkluderet er vigtige detaljer om de processer, der er nødvendige for at sikre cellelevedygtighed og fænotypespecificitet.

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Biologi udgave 170
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