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Effektiv Generation Menneskelige induceret pluripotente stamceller fra humane somatiske celler med Sendai-virus
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JoVE Journal Biology
Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Effektiv Generation Menneskelige induceret pluripotente stamceller fra humane somatiske celler med Sendai-virus

DOI:
10.3791/51406-v

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09:43 min

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April 23, 2014

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, ,
1Institute for Cell Engineering, Department of Neurology and Neuroscience,Johns Hopkins University School of Medicine

Chapters

  • 00:05Title
  • 01:17Transduction
  • 03:40Co-culturing
  • 06:01Expanding the Induced Pluripotent Stem Cells
  • 07:10Results: Induced Pluripotent Stem Cell Characterization
  • 09:12Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi vores etablerede metode til at omprogrammere humane somatiske celler i transgene-fri menneskelige iPSCs med Sendai-virus, som viser ensartet resultat og øget effektivitet.

Tags

Keywords: Human Induced Pluripotent Stem CellsIPSCsSendai-virusIntegration-freeTransgene-freeReprogrammingSomatic CellsGenetic DiseasesCell TherapyDrug ScreeningDisease ModelingHigh EfficiencyCost-effective

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