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Påvisning af SARS-CoV-2 neutraliserende antistoffer ved hjælp af fluorescerende billeddannelse af pseudovirusinfektion
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
DOI:

10:25 min

June 05, 2021

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Chapters

  • 00:04Introduction
  • 00:53Plating and Maintenance of Vero E6 Cells
  • 02:22Preparing the Vesicular Stomatitis Virus-Spike-EGFP (VSV-S-EGFP) Pseudovirus
  • 03:46Titering the VSV-S-eGFP Pseudovirus
  • 05:52Neutralizing SARS-CoV-2 Pseudovirus Using Convalescent Patient Serum and Commercial Antibodies
  • 09:07Results: SARS-CoV-2 Pseudovirus Neutralization by Commercial Antibodies and Convalescent Patient Serum
  • 10:00Conclusion

Summary

Automatic Translation

Den protokol, der er beskrevet her skitserer en hurtig og effektiv metode til måling neutraliserende antistoffer mod SARS-CoV-2 spike protein ved at evaluere evnen af rekonvalescerende serum prøver til at hæmme infektion med en forbedret grøn fluorescerende protein-mærket vesikulær stomatitis virus pseudotypeeret med spike glycoprotein.

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