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A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

DOI:
10.3791/62487-v

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07:08 min

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April 06, 2021

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, , ,
1Luminex Corporation, 2Departments of Pathology and Laboratory Medicine, Clinical Microbiology,University of Rochester Medical Center, 3Department of Microbiology and Immunology,University of Rochester Medical Center

Chapters

  • 00:05Introduction
  • 00:54Dual Reporter IgG and IgM Serological Assay
  • 04:08Dual Reporter Neutralization Assay
  • 04:53Results: Optimization of the Dual Reporter IgG and IgM Serological Assay and Neutralization Assay
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

A flow analysis system for bead-based multiplexed assays which provides a two-reporter readout was used for the development of multiplex serological and antibody neutralization assays that can simultaneously measure neutralizing IgG and IgM antibodies for SARS-CoV-2.

Tags

Keywords: SARS-CoV-2Neutralization AssayIgGIgMMultiplexBead-basedFlow AnalysisDual ReporterAntigen-specificAntibody IsotypingPost-translational ModificationsFree Versus Bound Drug FormsLuminex

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