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Immunology and Infection
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使用假病毒感染的高通量荧光成像检测SARS-CoV-2中和抗体
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
Please note that all translations are automatically generated.
Click here for the English version.
使用假病毒感染的高通量荧光成像检测SARS-CoV-2中和抗体
DOI:
10.3791/62486-v
•
10:25 min
•
June 05, 2021
•
Taylor R. Jamieson
2
,
Joanna Poutou
2
,
Ricardo Marius
2
,
Xiaohong He
,
Reza Rezaei
2
,
Taha Azad
2
,
Carolina S. Ilkow
2
1
Ottawa Hospital Research Institute
,
2
Department of Biochemistry, Microbiology and Immunology
,
University of Ottawa
Chapters
00:04
Introduction
00:53
Plating and Maintenance of Vero E6 Cells
02:22
Preparing the Vesicular Stomatitis Virus-Spike-EGFP (VSV-S-EGFP) Pseudovirus
03:46
Titering the VSV-S-eGFP Pseudovirus
05:52
Neutralizing SARS-CoV-2 Pseudovirus Using Convalescent Patient Serum and Commercial Antibodies
09:07
Results: SARS-CoV-2 Pseudovirus Neutralization by Commercial Antibodies and Convalescent Patient Serum
10:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里描述的方案概述了一种快速有效的方法来测量针对SARS-CoV-2刺突蛋白的中和抗体,方法是评估恢复期血清样品通过增强型绿色荧光蛋白标记的水泡性口炎病毒抑制感染的能力。
Tags
SARS-CoV-2
Neutralizing Antibodies
High-throughput
Fluorescent Imaging
Pseudovirus Infection
Vero E6 Cells
VSV Spike EGFP Pseudovirus
Viral Titer
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