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在微流体平台内开发 3D 组织人类心脏组织
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform
DOI:

10:42 min

June 15, 2021

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Chapters

  • 00:00Introduction
  • 01:05hCF Dissociation
  • 03:26CM Dissociation
  • 05:59Collagen Preparation
  • 06:59Hydrogel Mixture and Cell Preparation
  • 07:41Device Insertion
  • 09:32Results: Cardiac Tissue Formation
  • 10:13Conclusion

Summary

Automatic Translation

本协议的目的是解释和演示高度对齐的人类心脏组织的三维(3D)微流体模型的发展,该模型由干细胞衍生心肌细胞组成,与心脏成纤维细胞(CFs)在生物仿真、胶原蛋白基水凝胶中共同培养,用于心脏组织工程、药物筛查和疾病建模。

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