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在微流体平台内开发 3D 组织人类心脏组织
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Bioengineering
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JoVE Journal Bioengineering
Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform
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在微流体平台内开发 3D 组织人类心脏组织

DOI:
10.3791/62539-v

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10:42 min

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June 15, 2021

•

,
1School of Biological and Health Systems Engineering,Arizona State University, 2Biodesign Virginia G. Piper Center for Personalized Diagnostics,Arizona State University

Chapters

  • 00:00Introduction
  • 01:05hCF Dissociation
  • 03:26CM Dissociation
  • 05:59Collagen Preparation
  • 06:59Hydrogel Mixture and Cell Preparation
  • 07:41Device Insertion
  • 09:32Results: Cardiac Tissue Formation
  • 10:13Conclusion

Summary

Automatic Translation

Automatic Translation

本协议的目的是解释和演示高度对齐的人类心脏组织的三维（3D）微流体模型的发展，该模型由干细胞衍生心肌细胞组成，与心脏成纤维细胞（CFs）在生物仿真、胶原蛋白基水凝胶中共同培养，用于心脏组织工程、药物筛查和疾病建模。

Tags

Microfluidic PlatformCardiovascular DiseaseMyocardiumStem Cell-derived CardiomyocytesCardiac FibroblastsCell DissociationCell CultureCell Alignment

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