Journal
/
/
用于质谱分析的最佳切割温度化合物中嵌入的人组织制备
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis
DOI:

09:09 min

April 27, 2021

, ,

Chapters

  • 00:04Introduction
  • 00:54Preparation of Tissue-Drying Wicks
  • 01:39Tissue Washing for Sphingolipidomic Optimal Cutting Temperature-Compound (OCT) Removal
  • 02:41Tissue Weighing for Data Normalization
  • 04:12Lipid Extraction
  • 07:03Results: Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC ESI MS/MS) Analysis of Tissues Following OCT Removal
  • 08:36Conclusion

Summary

Automatic Translation

鞘脂是生物活性代谢物,在人类疾病中具有公认的作用。用质谱法表征组织改变可以揭示疾病病因学中的作用或确定治疗靶点。然而,用于生物储存库中冷冻保存的OCT化合物会干扰质谱分析。我们概述了使用LC-ESI-MS / MS分析嵌入OCT的人体组织中鞘脂的方法。

Related Videos

Read Article