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JoVE Journal Cancer Research
Udvidelse og berigelse af Gamma-Delta (γδ) T-celler fra aferesed humant produkt
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Udvidelse og berigelse af Gamma-Delta (γδ) T-celler fra aferesed humant produkt

Article DOI: 10.3791/62622-v • 11:51 min • September 22nd, 2021
September 22nd, 2021 •
1, 1, 2, 1,2, 1,2, 1,2
1Cell Therapy Facility, Moffitt Cancer Center and Research Institute, 2Department of Immunology, Moffitt Cancer Center and Research Institute

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Præsenteret er en protokol for udvidelsen af Gamma-Delta (γδ) T celle stof produkt. Lymfocytter er isoleret ved opstemthed og γδ-beriget med zoledronsyre og interleukin-2. Alpha-beta T-celler er udtømt ved hjælp af en klinisk-grade magnetisk adskillelse enhed. ΓΔ-cellerne er co-cultured med K562-afledte, kunstige antigen-præsentere celler og udvidet.

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Kræftforskning Gamma-Delta (γδ) T Cell Gamma-Delta T celle lægemiddelprodukt cGMP Celleterapi Zometa Zoledronic Acid genetisk modificeret K562 kunstig Antigen-præsentere Celler
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