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基于增强子的表达构建体在小鼠 大脑中的 AAV部署
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
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基于增强子的表达构建体在小鼠 大脑中的 AAV部署

DOI:
10.3791/62650-v

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09:59 min

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March 31, 2022

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, ,
1Department of Psychiatry and Behavioral Sciences,University of California, Davis, 2Department of Neurobiology, Physiology and Behavior,University of California, Davis

Chapters

  • 00:04Introduction
  • 00:39Enhancer Candidate Sequence Cloning into the AAV Vector Plasmid
  • 03:09Intracranially Inject rAAV-Packaged Plasmid into Neonatal Mice
  • 05:32Image and Analyze Brain Tissue Sections for Enhancer Activity
  • 08:10Results: AAV Deployment of Enhancer-Based Expression Constructs in Mouse Brain
  • 09:21Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一种新型基于rAAV的瞬时增强子 - 报告基因测定。该测定可用于在小鼠 大脑中诱导 增强子驱动的体内表达。

Tags

AAVEnhancerExpressionReporterConstructCloningPCRGibson CloningPlasmidVirus TiterIn VivoMouse Brain

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