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Journal
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Immunology and Infection
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单纯疱疹病毒的生长、净化和滴定
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
Please note that all translations are automatically generated.
Click here for the English version.
单纯疱疹病毒的生长、净化和滴定
DOI:
10.3791/62677-v
•
06:14 min
•
May 13, 2021
•
Hong-My Nguyen*
1
,
Naresh Sah*
1
,
Melissa R. M. Humphrey
,
Samuel D. Rabkin
,
Dipongkor Saha
1
Department of Immunotherapeutics and Biotechnology, Jerry H. Hodge School of Pharmacy
,
Texas Tech University Health Sciences Center
,
2
Molecular Neurosurgery Laboratory and the Brain Tumor Research Center, Department of Neurosurgery
,
Massachusetts General Hospital and Harvard Medical School
Chapters
00:04
Introduction
01:06
Oncolytic Herpes Simplex Virus (oHSV) Growth
02:46
Oncolytic Herpes Simplex Virus (oHSV) Purification
05:10
Results: Microscopic Analysis of Vero Cells Infected with oHSV
05:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在本手稿中,我们描述了一个简单的方法的生长,纯化和滴定肿瘤疱疹简单病毒的药物前使用。
Tags
Oncolytic Herpes Simplex Virus
Cancer Immunotherapy
Virus Propagation
Virus Purification
Virus Titration
Biosafety Level Two
Virus Stock
Anticancer Immune Response
Virus-based Cancer Immunotherapy
Wild-type Herpes Simplex Virus
Genetically Engineered Herpes Simplex Virus
T-150 Square Centimeter Flasks
DPBS
IFCS
DMEM
Cell Scraper
Centrifugation
Virus Buffer
Benzonase Nuclease
Virus Titer
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