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Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
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Immunology and Infection
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JoVE Journal Immunology and Infection
Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1

Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1

DOI:
10.3791/53046-v

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11:56 min

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August 24, 2015

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, , ,
1Center for Biochemistry,University of Cologne, 2Department of Dermatology,University of Cologne

Chapters

  • 00:05Title
  • 01:16Preparation of Instruments and Culture Medium
  • 02:28Preparation of Epidermal Sheets from Murine Newborn Skin or from Adult Tail Skin
  • 04:31Dissociation of Epidermis for FACS Analysis
  • 05:57Infection of Epidermal Sheets with Herpes Simplex Virus Type 1 and Visualization of Infected Cells
  • 09:36Results: Herpes Simplex Virus Infection of Murine Epidermal Sheets
  • 11:20Conclusion

Summary

Automatic Translation

Automatic Translation

The skin is one target tissue of the human pathogen herpes simplex virus type 1 (HSV-1). To explore the invasion route of HSV-1 into tissue, we established an ex vivo infection model of murine epidermal sheets which represent the outermost layer of skin.

Tags

Keywords: Ex VivoMurine EpidermisHerpes Simplex Virus Type 1HSV-1KeratinocytesPrimary InfectionLatent InfectionReactivationICP0Nectin-1HVEMCell-to-cell SpreadVirus Production

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