JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
オンコ分解性ヘルペスシンプレックスウイルスの増殖、精製、滴定
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
Please note that all translations are automatically generated.
Click here for the English version.
オンコ分解性ヘルペスシンプレックスウイルスの増殖、精製、滴定
DOI:
10.3791/62677-v
•
06:14 min
•
May 13, 2021
•
Hong-My Nguyen*
1
,
Naresh Sah*
1
,
Melissa R. M. Humphrey
,
Samuel D. Rabkin
,
Dipongkor Saha
1
Department of Immunotherapeutics and Biotechnology, Jerry H. Hodge School of Pharmacy
,
Texas Tech University Health Sciences Center
,
2
Molecular Neurosurgery Laboratory and the Brain Tumor Research Center, Department of Neurosurgery
,
Massachusetts General Hospital and Harvard Medical School
Chapters
00:04
Introduction
01:06
Oncolytic Herpes Simplex Virus (oHSV) Growth
02:46
Oncolytic Herpes Simplex Virus (oHSV) Purification
05:10
Results: Microscopic Analysis of Vero Cells Infected with oHSV
05:44
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本稿では、前臨床用の単純ヘルペスウイルスの増殖、精製、滴定の簡単な方法について述べる。
Tags
Oncolytic Herpes Simplex Virus
Cancer Immunotherapy
Virus Propagation
Virus Purification
Virus Titration
Biosafety Level Two
Virus Stock
Anticancer Immune Response
Virus-based Cancer Immunotherapy
Wild-type Herpes Simplex Virus
Genetically Engineered Herpes Simplex Virus
T-150 Square Centimeter Flasks
DPBS
IFCS
DMEM
Cell Scraper
Centrifugation
Virus Buffer
Benzonase Nuclease
Virus Titer
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
Epstein - Barrウイルスの成長形質転換リンパ芽球様細胞株の樹立
単純ヘルペスウイルスの待ち時間と再活性化の研究の主ニューロン培養システム
感染細胞のクロマチンからのインフルエンザウイルスのリボ核タンパク質複合体のアフィニティ精製
単純ヘルペスウイルス1型を持つマウス表皮
の
ex vivo
感染
治療薬にリンパ腫細胞の感度での役割を決定するために、好中球の単離と解析
組換えプロトタイプ フォーミー ウイルス インテグラーゼの浄化中にヌクレアーゼの混入の検出と除去
アセンブリおよびプロトタイプ フォーミー ウイルス Intasomes の精製
蛍光共焦点顕微鏡を用いた単純ヘルペス ウイルス 1 蛋白質の核-細胞質転流の時空間解析
全血豚で表されるコーディングおよび非コーディングの RNA クラスの識別
世代、増幅、および組換え呼吸器合胞体ウイルスの滴定
Read Article