Journal
/
Biology
/
Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

DOI:
10.3791/62718-v

•

06:06 min

•

July 22, 2021

•

, , ,
1Bone and Joint Center,Henry Ford Health System, 2Department of Orthopedic Surgery,Henry Ford Health System, 3Center for Molecular Medicine and Genetics,Wayne State University

Chapters

  • 00:05Introduction
  • 00:56Sample Processing
  • 01:54Hard Tissue Homogenization
  • 03:05Soft Tissue Homogenization
  • 04:20Results: Tissue Identification and RNA Quality Assessment
  • 05:27Conclusion

Summary

Automatic Translation

Automatic Translation

Primary tissues obtained from patients following total knee arthroplasty provide an experimental model for osteoarthritis research with maximal clinical translatability. This protocol describes how to identify, process, and isolate RNA from seven unique knee tissues to support mechanistic investigation in human osteoarthritis.

Tags

RNA ExtractionOsteoarthritisHuman Knee JointGene ExpressionHistological AnalysisCartilageBoneMeniscusInfrapatellar Fat PadAnterior Cruciate LigamentSynoviumVastus Medialis Oblique MuscleLiquid NitrogenHomogenizationMortar And PestleAcid Guanidinium Phenol Solution

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region

RNA Extraction from Neuroprecursor Cells Using the Bio-Rad Total RNA Kit

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

Extraction and Analysis of Cortisol from Human and Monkey Hair

RNA Isolation from Mouse Pancreas: A Ribonuclease-rich Tissue

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues

Collection of Skeletal Muscle Biopsies from the Superior Compartment of Human Musculus Tibialis Anterior for Mechanical Evaluation

Generation of Human Kidney Tubuloids from Tissue and Urine

Read Article