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Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
JoVE Journal
Biology
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JoVE Journal Biology
Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
DOI:

06:06 min

July 22, 2021

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Chapters

  • 00:05Introduction
  • 00:56Sample Processing
  • 01:54Hard Tissue Homogenization
  • 03:05Soft Tissue Homogenization
  • 04:20Results: Tissue Identification and RNA Quality Assessment
  • 05:27Conclusion

Summary

Automatic Translation

Primary tissues obtained from patients following total knee arthroplasty provide an experimental model for osteoarthritis research with maximal clinical translatability. This protocol describes how to identify, process, and isolate RNA from seven unique knee tissues to support mechanistic investigation in human osteoarthritis.

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