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Biochemistry

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JoVE Journal Biochemistry
Studies van Chaperone-Cochaperone Interacties met behulp van Homogene Bead-Based Assay
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Studies van Chaperone-Cochaperone Interacties met behulp van Homogene Bead-Based Assay

Article DOI: 10.3791/62762-v • 06:51 min • July 21st, 2021
July 21st, 2021 •
1, 1, 1,2, 1,3, 1
1Department of Neuroscience Care and Society, Division of Neurogeriatrics, Karolinska Institutet, 2Department of Pharmaceutical Engineering & Technology, Indian Institute of Technology (BHU), 3Theme Inflammation and Aging, Karolinska University Hospital

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Dit protocol presenteert een techniek voor het onderzoeken van eiwit-eiwitinteracties met behulp van glutathion-gekoppelde donorkralen met GST-gefuseerde TPR-motief co-chaperones en acceptor kralen in combinatie met een Hsp90-afgeleid peptide. We hebben deze techniek gebruikt om kleine moleculen te screenen om Hsp90-FKBP51- of Hsp90-FKBP52-interacties te verstoren en hebben krachtige en selectieve Hsp90-FKBP51-interactieremmers geïdentificeerd.

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Biochemie AlphaScreen eiwit-eiwit interacties moleculaire chaperonne Hsp90 FKBP51 FKBP52 TPR eiwitten compound screening
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