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Journal / Developmental Biology / Dybe og rumligt kontrollerede volumen ablations…
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JoVE Journal Developmental Biology
Dybe og rumligt kontrollerede volumen ablations ved hjælp af en to-Foton Mikroskop i Zebrafish Gastrula
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Dybe og rumligt kontrollerede volumen ablations ved hjælp af en to-Foton Mikroskop i Zebrafish Gastrula

Article DOI: 10.3791/62815-v • 09:50 min • July 15th, 2021
July 15th, 2021 •
1, 1, 1
1Laboratory for Optics and Biosciences, CNRS UMR7645, INSERM U1182, Institut Polytechnique de Paris

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Embryonal udvikling kræver storstilet koordinering af cellebevægelse. To-foton excitation medieret laser ablation tillader rumligt kontrolleret 3-dimensionelle ablation af store grupper af dybe celler. Derudover kan denne teknik sondere reaktionen af kollektivt migrerende celler in vivo til forstyrrelser i deres mekaniske miljø.

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Udviklingsbiologi udgave 173
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