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Transposoninnsetting Sekvensering som et verktøy for å belyse bakteriekoloniseringsfaktorer i en Burkholderia gladioli Symbiont av Lagria villosa Beetles
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Transposoninnsetting Sekvensering som et verktøy for å belyse bakteriekoloniseringsfaktorer i en Burkholderia gladioli Symbiont av Lagria villosa Beetles

Article DOI: 10.3791/62843-v • 09:55 min • August 12th, 2021
August 12th, 2021 •
1, 1,2, 1,3
1Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution, Johannes Gutenberg University, 2Department of Insect Symbiosis, Max Planck Institute for Chemical Ecology, 3Department of Plant and Environmental Sciences, Section for Organismal Biology, University of Copenhagen

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Dette er en tilpasset metode for å identifisere kandidatens insektkoloniseringsfaktorer i en Burkholderia gunstig symbiont. Billeverten er infisert med et tilfeldig mutantbibliotek generert via transposonmutagenese, og bibliotekets kompleksitet etter kolonisering sammenlignes med en kontroll dyrket in vitro.

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Biologi Utgave 174 Burkholderia Symbiont Vertskolonisering Transposonsekvensering Tn5 transposon Lagria villosa
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