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JoVE Journal Cancer Research
Analytische Bestimmung der mitochondrialen Funktion von exzidierten soliden Tumorhomogenaten
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Analytische Bestimmung der mitochondrialen Funktion von exzidierten soliden Tumorhomogenaten

Article DOI: 10.3791/62875-v • 11:32 min • August 6th, 2021
August 6th, 2021 •
1,2,3, 1, 2,4, 1,3
1Integrated Physiology and Molecular Medicine Laboratory, Pennington Biomedical Research Center, 2Clinical Oncology and Metabolism, Pennington Biomedical Research Center, 3Department of Nutrition, Case Western Reserve University, 4Department of Clinical Nutrition, University of Texas Southwestern Medical Center

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Wir entwickelten ein praktisches Protokoll und einen analytischen Ansatz zur Bewertung der mitochondrialen oxidativen Phosphorylierung und Der Elektronentransferkapazität in frischen Tumorhomogenaten. Dieses Protokoll kann leicht angepasst werden, um verschiedene mitochondriale Funktionen zu überwachen, die zur Entstehung, Progression und Ansprechen auf die Behandlung von Krebs beitragen.

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Krebsforschung Ausgabe 174
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