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Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones
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Chapters
- 00:05Title
- 01:42Tissue Dissection, Post-fixation, and Sectioning
- 05:37Immunostaining: Floating Section Staining for Plantar Punch Tissue
- 07:17Immunostaining: On-slide Staining for Limb Bone Sections
- 08:38Epifluorescence and Confocal Imaging of Immunostained Plantar Punch and Bone Tissue
Immunocytochemical identification of peripheral sensory nerve fiber subtypes (and detection of protein expression therein) are key to the understanding of molecular mechanisms underlying peripheral sensation. Here we describe methods for preparation of peripheral/visceral tissue samples, such as skin and limb bones, for specific immunostaining of peripheral sensory nerve fibers.
