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Simultaneous Cryosectioning of Multiple Rodent Brains
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Neuroscience
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JoVE Journal Neuroscience
Simultaneous Cryosectioning of Multiple Rodent Brains

Simultaneous Cryosectioning of Multiple Rodent Brains

DOI:
10.3791/58513-v

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06:37 min

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September 18, 2018

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, , , ,
1Department of Child Health,University of Arizona College of Medicine, 2Department of Biological Sciences,University of Bath, 3BARROW Neurological Institute at Phoenix Children’s Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences,University of Arizona College of Medicine

Chapters

  • 00:04Title
  • 00:44Cryoprotection of Perfused Brains and Brain Freezing
  • 03:18Preparing the Megabrain for Sectioning on the Cryostat
  • 04:14Cryosectioning the Megabrain
  • 05:24Results: Freezing and Sectioning Brain Tissue from Multiple Animals with Megabrain
  • 05:54Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to freeze and section brain tissue from multiple animals as a timesaving alternative to processing single brains. This reduces staining variability during immunohistochemistry and reduces time cryosectioning and imaging.

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Keywords: Simultaneous CryosectioningMultiple Rodent BrainsImmunohistochemistryTime-saving MethodCryosectionEmbedding MoldIsopentaneOptimal Cutting TemperatureOCT CompoundTranscardial PerfusionParaformaldehydeSucrose SolutionCerebellumOlfactory BulbsBrain Hemispheres

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