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06:37 min
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September 18, 2018
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Summary
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Here, we present a protocol to freeze and section brain tissue from multiple animals as a timesaving alternative to processing single brains. This reduces staining variability during immunohistochemistry and reduces time cryosectioning and imaging.
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Cite this Article
Green, T. R., Ortiz, J. B., Harrison, J. L., Lifshitz, J., Rowe, R. K. Simultaneous Cryosectioning of Multiple Rodent Brains. J. Vis. Exp. (139), e58513, doi:10.3791/58513 (2018).
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