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乳腺上皮和内皮细胞球状体作为乳腺癌研究的潜在功能 体外 模型
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Cancer Research
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JoVE Journal Cancer Research
Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research
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乳腺上皮和内皮细胞球状体作为乳腺癌研究的潜在功能 体外 模型

DOI:
10.3791/62940-v

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08:30 min

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July 12, 2021

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, , , , , ,
1Department of Reproductive Endocrinology, Wagistrasse 14,University of Zurich and University Hospital Zurich, 2Department of Dermatology, Wagistrasse 18,University of Zurich and University Hospital Zurich, 3Department of Pharmacology and Chemical Biology,University of Pittsburgh

Chapters

  • 00:04Introduction
  • 00:49Spheroid Formation
  • 03:55Spheroid Immunohistochemistry Study
  • 05:24Live/Dead Staining in Spheroids
  • 05:56Results: Generation of Mammary Epithelial and Endothelial Cell Spheroids
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

乳腺上皮细胞和内皮细胞之间的串扰对乳腺癌进展、肿瘤生长和转移有重要贡献。在这项研究中，球状体是由乳腺癌细胞与血管和/或淋巴内皮细胞一起制成的，并证明了它们作为乳腺癌研究 的体外 系统的适用性。

Tags

Breast CancerCell Spheroids3D ModelMammary Epithelial CellsEndothelial CellsCancer Cell-endothelial Cell InteractionsProliferationMetastasisIn Vitro ModelCell DistributionDye StainingEnzymatic DetachmentCell CountingCell SuspensionCell Co-culture

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