/

/

Enhancing Tumor Content through Tumor Macrodissection

JoVE Journal
Biology
Author Produced

This content is Free Access.

JoVE Journal Biology

Enhancing Tumor Content through Tumor Macrodissection

Enhancing Tumor Content through Tumor Macrodissection

DOI:

10.3791/62961-v

•

10:04 min

•

February 12, 2022

•

Lee Wisner, Brandon Larsen, Alanna Maguire

¹Department of Research,Mayo Clinic, ²Department of Laboratory Medicine and Pathology,Mayo Clinic

Chapters

00:05Introduction
01:08Sample Preparation
02:47Pathology Review
03:49Deparaffinization
05:00Macrodissection
07:04Results
09:43Conclusions

Summary

Automatic Translation

This protocol presents a method to increase the percent tumor content of formalin-fixed paraffin-embedded tissue samples.

Tags

Keywords: Tumor Macrodissection Tumor Content Non-tumor Material Genomic Analysis RNA And DNA Extraction Paraffin Blocks Microtome Hematoxylin And Eosin Staining Pathology Review

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity

Generation of Human CD40-activated B cells

An In vitro FluoroBlok Tumor Invasion Assay

Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish

A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells

Isolation of Immune Cells from Primary Tumors

A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

Read Article