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Journal / Bioengineering / Mikrotubuli Plus-End Dynamics Visualisierung im…
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Bioengineering
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JoVE Journal Bioengineering
Mikrotubuli Plus-End Dynamics Visualisierung im Huntington-Modell basierend auf humanen primären Hautfibroblasten
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Mikrotubuli Plus-End Dynamics Visualisierung im Huntington-Modell basierend auf humanen primären Hautfibroblasten

Article DOI: 10.3791/62963-v • 10:38 min • January 8th, 2022
January 8th, 2022 •
*1,2, *2,3, 1,2, 3,4, 3,4, 1,3
1A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
* These authors contributed equally

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Dieses Protokoll ist der Mikrotubuli-Plus-End-Visualisierung durch EB3-Proteintransfektion gewidmet, um ihre dynamischen Eigenschaften in der primären Zellkultur zu untersuchen. Das Protokoll wurde an menschlichen primären Hautfibroblasten implementiert, die von Huntington-Patienten gewonnen wurden.

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Bioengineering Ausgabe 179 Mikrotubuli Transfektion EB3 Primärkultur Huntington-Krankheit Lebenszeitmikroskopie
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