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Journal / Bioengineering / Визуализация динамики микротрубочек Plus-End в…
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Bioengineering
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JoVE Journal Bioengineering
Визуализация динамики микротрубочек Plus-End в модели болезни Гентингтона на основе первичных фибробластов кожи человека
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Визуализация динамики микротрубочек Plus-End в модели болезни Гентингтона на основе первичных фибробластов кожи человека

Article DOI: 10.3791/62963-v • 10:38 min • January 8th, 2022
January 8th, 2022 •
*1,2, *2,3, 1,2, 3,4, 3,4, 1,3
1A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
* These authors contributed equally

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Этот протокол предназначен для визуализации микротрубочек плюс конец путем трансфекции белка EB3 для изучения их динамических свойств в первичной клеточной культуре. Протокол был реализован на первичных фибробластах кожи человека, полученных от пациентов с болезнью Гентингтона.

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Биоинженерия выпуск 179 микротрубочки трансфекция EB3 первичная культура болезнь Гентингтона пожизненная микроскопия
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