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Journal / Bioengineering / Visualización dinámica de microtúbulos plus-end…
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Bioengineering
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JoVE Journal Bioengineering
Visualización dinámica de microtúbulos plus-end en el modelo de la enfermedad de Huntington basado en fibroblastos primarios de piel humana
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Visualización dinámica de microtúbulos plus-end en el modelo de la enfermedad de Huntington basado en fibroblastos primarios de piel humana

Article DOI: 10.3791/62963-v • 10:38 min • January 8th, 2022
January 8th, 2022 •
*1,2, *2,3, 1,2, 3,4, 3,4, 1,3
1A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
* These authors contributed equally

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Este protocolo está dedicado a la visualización de microtúbulos plus-end mediante transfección de proteínas EB3 para estudiar sus propiedades dinámicas en cultivo celular primario. El protocolo se implementó en fibroblastos primarios de piel humana obtenidos de pacientes con enfermedad de Huntington.

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Bioingeniería Número 179 microtúbulos transfección EB3 cultivo primario enfermedad de Huntington microscopía de por vida
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