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Journal / Bioengineering / Visualizzazione dinamica plus-end dei microtubuli …
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Bioengineering
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JoVE Journal Bioengineering
Visualizzazione dinamica plus-end dei microtubuli nel modello della malattia di Huntington basato sui fibroblasti cutanei primari umani
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Visualizzazione dinamica plus-end dei microtubuli nel modello della malattia di Huntington basato sui fibroblasti cutanei primari umani

Article DOI: 10.3791/62963-v • 10:38 min • January 8th, 2022
January 8th, 2022 •
*1,2, *2,3, 1,2, 3,4, 3,4, 1,3
1A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
* These authors contributed equally

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Questo protocollo è dedicato alla visualizzazione plus-end dei microtubuli mediante trasfezione della proteina EB3 per studiarne le proprietà dinamiche in coltura cellulare primaria. Il protocollo è stato implementato su fibroblasti cutanei primari umani ottenuti da pazienti affetti da Malattia di Huntington.

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Bioingegneria Numero 179 microtubuli trasfezione EB3 coltura primaria Malattia di Huntington microscopia a vita
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