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Journal / Bioengineering / Isolamento e Cultura das Células Epiteliais…
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JoVE Journal Bioengineering
Isolamento e Cultura das Células Epiteliais Gengivais Humanas Primárias usando Y-27632
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Isolamento e Cultura das Células Epiteliais Gengivais Humanas Primárias usando Y-27632

Article DOI: 10.3791/62978-v • 06:47 min • November 6th, 2021
November 6th, 2021 •
1,2,3, 4, 3, 1, 1, 1, 1, 1, 1, 2,5, 1
1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 3Department of Stomatology, Shengli Oilfield Central Hospital, 4Department of Pediatric Surgery, Shengli Oilfield Central Hospital, 5Ningbo Stomatology Hospital of Savaid Stomatology School, Hangzhou Medical College

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Aqui apresentamos um método modificado para o isolamento e cultura das células epiteliais gengival humanas adicionando o inibidor de Rocha, Y-27632, ao método tradicional. Este método é mais fácil, menos demorado, melhora as propriedades das células-tronco e produz um número maior de células epiteliais de alto potencial, tanto para o laboratório quanto para aplicações clínicas.

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Bioengenharia Edição 177 células epiteliais gengivais método enzimático método de explantamento direto isolamento celular cultura celular Y-27632 inibidor de quinase associado a Rho análise de imunofluorescência
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