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Area-based Image Analysis Algorithm for Quantification of Macrophage-fibroblast Cocultures
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Area-based Image Analysis Algorithm for Quantification of Macrophage-fibroblast Cocultures
DOI:

07:05 min

February 15, 2022

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Chapters

  • 00:04Introduction
  • 00:39Cell Culture and Image Acquisition
  • 02:09Monoculture Image Analysis
  • 03:58Coculture Image Analysis
  • 05:05Results: Area-Based Image Analysis Algorithm of Macrophage Fibroblast Cocultures
  • 06:26Conclusion

Summary

Automatic Translation

We present a method, which utilizes a generalizable area-based image analysis approach to identify cell counts. Analysis of different cell populations exploited the significant cell height and structure differences between distinct cell types within an adaptive algorithm.

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