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Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images
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Chapters
- 00:00Introduction
- 01:06Download Icy and the “Substructure Analyzer” Protocol on Icy Website
- 01:30Opening the Protocol
- 02:03Interacting with the Workflow on Icy
- 02:49Merging of the Channels of an Image
- 04:57Segmentation of the Regions of Interest
- 07:42Fluorescent Signal Detection and Analysis
- 09:25Run the Protocol
- 10:35Representative Results
- 13:48Conclusion
We present a freely available workflow built for rapid exploration and accurate analysis of cellular bodies in specific cell compartments in fluorescence microscopy images. This user-friendly workflow is designed on the open-source software Icy and also uses ImageJ functionalities. The pipeline is affordable without knowledge in image analysis.
