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JoVE Journal Neuroscience
Live imaging av mitokondriell glutation Redox tillstånd i primära nervceller med hjälp av en ratiometrisk indikator
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Live imaging av mitokondriell glutation Redox tillstånd i primära nervceller med hjälp av en ratiometrisk indikator

Article DOI: 10.3791/63073-v • 07:47 min • October 20th, 2021
October 20th, 2021 •
1, 1, 2, 1
1Department of Medical Cell Biology, Institute for Anatomy and Cell Biology, Heidelberg University, 2Department of Neurogenetics, Max-Planck-Institute for Experimental Medicine

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Denna artikel beskriver ett protokoll för att bestämma skillnader i basal redox tillstånd och redox svar på akuta störtfloder i primära hippocampal och när nervceller med confocal levande mikroskopi. Protokollet kan tillämpas på andra celltyper och mikroskop med minimala modifieringar.

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Neurovetenskap nummer 176 Grx1-roGFP2 konfokal mikroskopi oxidativ stress hippocampala nervceller excitotoxicitet mitokondriell membranpotential
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