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JoVE Journal Neuroscience
In vivo Kalciumavbildning av neuronala ensembler i nätverk av primära sensoriska neuroner i intakta dorsala rotganglier
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In vivo Kalciumavbildning av neuronala ensembler i nätverk av primära sensoriska neuroner i intakta dorsala rotganglier

Article DOI: 10.3791/64826-v • 09:07 min • February 10th, 2023
February 10th, 2023 •
1, 1, 1, 1, 1,2
1Department of Oral & Maxillofacial Surgery, School of Dentistry, University of Texas Health Science Center at San Antonio, 2Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, Radiological Sciences, University of Texas Health Science Center at San Antonio

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Detta protokoll beskriver den kirurgiska exponeringen av dorsalrotganglion (DRG) följt av GCaMP3 (genetiskt kodad Ca2+ indikator; Grönt fluorescerande protein-kalmodulin-M13-protein 3) Ca2+ avbildning av neuronensemblerna med hjälp av Pirt-GCaMP3-möss samtidigt som man applicerar en mängd olika stimuli på den ipsilaterala baktassen.

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