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1Department of Cardiovascular & Metabolic Sciences, Lerner Research Institute,Cleveland Clinic, 2University Hospitals Cleveland Medical Center, 3Case Western Reserve University, 4Center for Microbiome & Human Health,Cleveland Clinic, 5Electron Microscopy Core, Lerner Research Institute,Cleveland Clinic
Chapters
- 00:05Introduction
- 01:09Culturing Conditions and Extracellular Vesicles Isolation
- 05:15Extracellular Vesicles (EVs) Isolation with Size Exclusion Chromatography (SEC)
- 07:15EV Preparation Quality Control and Storage
- 07:58Results: Analysis of EVs Fraction Collected from Escherichia coli MP1
- 09:24Conclusion
Bakterier utskiller nanometerstore ekstracellulære vesikler (EV) som bærer bioaktive biologiske molekyler. EV-forskning fokuserer på å forstå deres biogenese, rolle i mikrobe-mikrobe og vertsmikrobe-interaksjoner og sykdom, samt deres potensielle terapeutiske anvendelser. En arbeidsflyt for skalerbar isolering av elbiler fra ulike bakterier presenteres for å lette standardisering av EV-forskning.
