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Biochemistry

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JoVE Journal Biochemistry
In vitro Drugsscreening tegen alle levenscyclusstadia van Trypanosoma cruzi met behulp van parasieten die β-galactosidase tot expressie brengen
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In vitro Drugsscreening tegen alle levenscyclusstadia van Trypanosoma cruzi met behulp van parasieten die β-galactosidase tot expressie brengen

Article DOI: 10.3791/63210-v • 08:48 min • November 5th, 2021
November 5th, 2021 •
1,2, 1, 1, 3, 1,2, 1,2
1Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), 2Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, 3Instituto de Investigaciones Biomédicas (INBIOMED), Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

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We beschrijven een high-throughput colorimetrische test die β-galactosidase-activiteit meet in drie levenscyclusstadia van Trypanosoma cruzi, de veroorzaker van de ziekte van Chagas. Deze test kan worden gebruikt om trypanocidale verbindingen op een eenvoudige, snelle en reproduceerbare manier te identificeren.

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Biochemie Nummer 177
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