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Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei
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Biochemistry
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JoVE Journal Biochemistry
Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

DOI:
10.3791/58334-v

•

08:44 min

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January 22, 2019

•

,
1Biology Centre,Czech Academy of Science, Institute of Parasitology, 2Faculty of Science,University of South Bohemia

Chapters

  • 00:04Title
  • 00:57Preparation of Sub-Mitochondrial Particles
  • 02:43Chloroform Release of F1-ATPase from the Membrane
  • 04:08Anion-Exchange Chromatography
  • 05:34Size-Exclusion Chromatography
  • 06:49Results: Two-Step Purification Results in Effective Isolation of F1-ATPase
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the purification of F1-ATPase from the cultured insect stage of Trypanosoma brucei. The procedure yields a highly pure, homogeneous, and active complex suitable for structural and enzymatic studies.

Tags

Keywords: F1-ATPaseTrypanosoma BruceiMitoplastsProtein PurificationMitochondrial MembraneSonicationUltracentrifugationChloroform ExtractionDounce Homogenizer

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