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Journal / Biochemistry / In vitro Lægemiddelscreening mod alle…
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Biochemistry

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JoVE Journal Biochemistry
In vitro Lægemiddelscreening mod alle livscyklusfaser af Trypanosoma cruzi ved hjælp af parasitter, der udtrykker β-galactosidase
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In vitro Lægemiddelscreening mod alle livscyklusfaser af Trypanosoma cruzi ved hjælp af parasitter, der udtrykker β-galactosidase

Article DOI: 10.3791/63210-v • 08:48 min • November 5th, 2021
November 5th, 2021 •
1,2, 1, 1, 3, 1,2, 1,2
1Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), 2Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, 3Instituto de Investigaciones Biomédicas (INBIOMED), Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

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Vi beskriver et kolorimetrisk assay med høj kapacitet, der måler β-galactosidaseaktivitet i tre livscyklusfaser af Trypanosoma cruzi, det forårsagende middel til Chagas sygdom. Dette assay kan bruges til at identificere trypanocidale forbindelser på en nem, hurtig og reproducerbar måde.

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Biokemi udgave 177
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