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電気生理学的研究のための成体、幼若、幼虫および胚性ゼブラフィッシュからの心臓および血管平滑筋細胞の単離(英語)
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Biology
Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies
Please note that all translations are automatically generated.
Click here for the English version.
電気生理学的研究のための成体、幼若、幼虫および胚性ゼブラフィッシュからの心臓および血管平滑筋細胞の単離(英語)
DOI:
10.3791/63225-v
•
07:04 min
•
February 09, 2022
•
Soma S. Singareddy
,
Conor McClenaghan
,
Helen I. Roessler
,
Robert Tryon
,
Colin G. Nichols
1
Department of Cell Biology and Physiology and Center for the Investigation of Membrane Excitability Diseases
,
Washington University in St. Louis
,
2
Department of Genetics, Center for Molecular Medicine
,
University Medical Center Utrecht, Utrecht University
Chapters
00:05
Introduction
00:42
Isolation of Atrium, Ventricle, and Bulbous Arteriosus from Adult, Juvenile, and Larval Zebrafish
01:37
Dissociation of Cardiomyocytes from Adult, Juvenile, and Larval Zebrafish for Electrophysiological Studies
02:59
Dissociation of Vascular Smooth Muscle Cells from Adult and Juvenile Zebrafish for Electrophysiological Studies
04:14
Isolation of Hearts from Embryonic Zebrafish for Electrophysiological Studies
05:21
Results: Action Potential Properties from Isolated Zebrafish Cardiomyocytes
Summary
Automatic Translation
English (Original)
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Automatic Translation
本プロトコルは、電気生理学的研究に適した、成体、幼若、幼虫、および胚性ゼブラフィッシュ(
Danio rerio
)からの生存可能な心臓および血管平滑筋細胞の急性単離を記載している。
Tags
Cardiac Myocytes
Vascular Smooth Muscle Cells
Zebrafish
Electrophysiology
Patch Clamp
Cell Isolation
Perfusion Buffer
Digestion Buffer
Stopping Buffer
Centrifugation
Trituration
Article
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