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電気生理学的研究のための成体、幼若、幼虫および胚性ゼブラフィッシュからの心臓および血管平滑筋細胞の単離(英語)
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies
DOI:

07:04 min

February 09, 2022

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Chapters

  • 00:05Introduction
  • 00:42Isolation of Atrium, Ventricle, and Bulbous Arteriosus from Adult, Juvenile, and Larval Zebrafish
  • 01:37Dissociation of Cardiomyocytes from Adult, Juvenile, and Larval Zebrafish for Electrophysiological Studies
  • 02:59Dissociation of Vascular Smooth Muscle Cells from Adult and Juvenile Zebrafish for Electrophysiological Studies
  • 04:14Isolation of Hearts from Embryonic Zebrafish for Electrophysiological Studies
  • 05:21Results: Action Potential Properties from Isolated Zebrafish Cardiomyocytes

Summary

Automatic Translation

本プロトコルは、電気生理学的研究に適した、成体、幼若、幼虫、および胚性ゼブラフィッシュ(Danio rerio)からの生存可能な心臓および血管平滑筋細胞の急性単離を記載している。

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