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Högupplöst tredimensionell avbildning av fotplattan Vasculature i en Murine Hindlimb Gangrene Modell
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Högupplöst tredimensionell avbildning av fotplattan Vasculature i en Murine Hindlimb Gangrene Modell

Article DOI: 10.3791/63284-v • 08:16 min • March 16th, 2022
March 16th, 2022 •
1, 1,2, 3, 1, 1,2, 4, 1,2, 1,2, 1,2
1Dewitt Daughtry Family Department of Surgery, University of Miami Miller School of Medicine, 2Vascular Biology Institute, University of Miami Miller School of Medicine, 3University of Miami, 4Analytical Imaging Core Facility, University of Miami

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Detta protokoll beskriver en unik, kliniskt relevant modell av perifera kranskärlens sjukdom som kombinerar femorala gatan och ven elektrokoagulering med administrering av en kväveoxid syntas inhibitor att inducera hindlimb kallbrand i FVB möss. Intracardiac DiI perfusion används sedan för högupplöst, tredimensionell avbildning av fotplattan vaskulatur.

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Medicin nummer 181 Hindlimb ischemi kallbrand femorala arteriell ligatur L-NAME DiI perfusion perifer arteriell sjukdom neovaskulärisering angiogenes
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